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Before imaging, animals were washed 3 with M9 to remove any residual dye on the outer cuticle of the animal

Immunohistochemical studies in the brains of scrapie-infected mice have revealed the presence of lipid oxidation markers, nitrotyrosine (a marker of peroxynitrite production), and heme-oxygenase-1 (an enzyme leading to the development of antioxidant molecules), suggesting that oxidative stress might be one mechanism of neuronal loss [131, 132]

The placenta experiences a heightened level of OS in certain pathologic conditions of pregnancies, including gestational diabetes, fetal growth restriction, preeclampsia and miscarriage [64,65,66]

Quantitative real-time PCR Cells and tissues were first lysed using Trizol, then the total RNA was extracted with RNA Easy Fast Tissue/Cell Kit (Tiangen, city, ST, Ctry

12 Because its an antioxidant, its a vital mineral to help protect cells from the oxidative stress and inflammation that can lead to illness and disease