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Description
Generation of monoclonal HEK293T knockout cell lines Semiconfluent HEK293T cells in 6-well plates were transfected with 2.5g of a pSpCas9(BB)-2A-EGFP plasmid containing either DCAF16 or FBXO22 sgRNA using Lipofectamine 2000 (Thermo Fisher Scientific, 11668027)
When a heavy metal enters a cell, it looks for places to attach
CGRP binds to CLR receptors on osteoblast cell surfaces, directly enhancing osteogenic gene expression
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coli , we targeted roGFP2 to this compartment
