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The samples were added to the working solution and incubated at room temperature for 5 min, followed by the addition of the configured NADPH reserve solution
These motions are consistent with the loss of key interactions mediated by Tyr 37 of amylin peptides and the poor resolution of the RAMP3 ECD in the 3D EM map reconstructions
The DPPH scavenging activity assay determined the antioxidants' ability to neutralize DPPH radicals by donating electrons, resulting in a color shift measured with a spectrophotometer 65
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