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Insufficient B12 in the body raises susceptibility to infections and diseases, and is very harmful to cat's nervous system, in turn leads to faster onset of neurological symptoms, and severe or irreversible nervous system damages

The lux reaction requires reducing co-factors (NAD(P)H and FMNH 2 ), oxygen, and ATP, thus tightly coupling it to cellular metabolism 32

Accessed April 30, 2021

Box 2 Factors influencing the accuracy of results of serum B 12 and related biomarkers Considerable variability exists between the different commercially available serum B 12 assays.31 Day-to-day variation of serum B 12 may occur

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