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At the cellular level, in vitro assays using cell lines (e.g., HEK293) transfected to express specific receptors like GLP-1R or MC4R are essential for characterizing ligand binding affinities, potency (EC50), and downstream signaling events like cAMP accumulation

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doi: 10.1177/001872089103300504
397 In the nematode Caenorhabditis elegans , overexpression of ATGL or muscle-specific activation of protein kinase A extends lifespan
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