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The plasmid was microinjected into fertilized eggs, and positive transgenic rats were confirmed by PCR amplification of an 1112 bp fragment using MHC -promoter-F (5 TGTAGACAGCAGATCACGATTCTC) and MHC -h- Cyp2e1 -R (5 ATTTCCACGAGCAGGCAGTC) primers with 30 cycles of 30 s at 94 C, 40 s at 58 C, and 60s at 72 C

In addition, due to the long-acting properties of YEZTUGO, alternative forms of PrEP should be considered following discontinuation of YEZTUGO for those individuals with HIV-1 negative status who are at continuing risk of HIV-1 acquisition and initiated within 28 weeks of the last YEZTUGO injection [see Warnings and Precautions (5.3)]

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Protein supplementation: Shakes and supplements help reach targets when whole food intake is limited

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