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For the NF-B investigation, some cochlear paraffin sections were obtained, stained for NF-B using rabbit polyclonal NF-_B p65 (phospho S276) primary antibody (ab194726), goat anti-rabbit IgG H&L (HRP) secondary antibody (ab205718) (Abcam, Cambridge, UK), and 3,3-diaminobenzidine chromogen following the ABC technique (Ramos-Vara et al