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Cytochrome b5 reductases: redox regulators of cell homeostasis
Lipid measurements using LCMS For lipid extraction, samples were mixed with 20 C isopropanol (150 l per 5 l serum and 40 l per mg tissue), vortexed and immediately centrifuged at 16,000 g for 10 min at 4 C 15
No obvious conserved features in the recognition of sterol ligands are observed for these GPCRs and SRD5A2, suggesting highly diverse mechanisms of sterol ligand recognition by membrane proteins
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