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In intestinal tissues from mice the I/R model, the levels of pro-ferroptotic factors, such as iron and ACSL4 are increased, while those of anti-ferroptotic factors, such as GPX4, ferritin heavy chain 1 (FTH1) and GSH are decreased, with the inhibition of ferroptosis ameliorating intestinal injury and barrier dysfunction (68)

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The homogenate was filtered through a 100-M cell strainer and centrifuged at 1,000 g for 10 min at 4 C, then the nuclear pellet was washed once in nucleus preparation buffer followed by resuspension in PBS with 1% BSA and 0.2 U l Roche Protector RNase inhibitor