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Trays are then sealed and stored in a clean area until use

Cells were harvested and lysed in lysis buffer (50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 10 mM imidazole, and 2 mM PMSF) using a Pulse 150 Ultrasonic Homogenizer with 6 probe (Benchmark Scientific, Sayreville, NJ)
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WPBs are endothelial granules that store coiled vWF multimers

The third factor is electrolyte depletion