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Low cultivation temperatures can also reduce or impair protein degradation due to a poor activity of heat shock proteases that are usually induced during protein overproduction in E

Proteins in lipid raft (LR) fractions were isolated by centrifugation at 13 000 x g, 4C, for 15 minutes

BOX 4.1

Liu R, Paxton WA, Choe S, Ceradini D, Martin SR, Horuk R et al
