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doi: 10.1080/01635581.2013.776693

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The results indicate that GLSAS interacts with the cMyc pathway and modulates GLS production to influence PDAC metabolism

Cell signaling assays For analysis of human LepR mutants, HEK-293T cells were plated in six-well culture dishes coated with fibronectin (Millipore) at 0.7 10 6 cells per well in DMEM (Dulbeccos Modified Eagle Medium) supplemented with 10% v/v fetal bovine serum, penicillin-streptomycin, 1 mM sodium pyruvate, 10 nM HEPES and 2 mM GlutaMAX TM (Gibco)

It is the difference between a medically supervised treatment and a gamble with an unregulated product
