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A subsequent study also supported the hypothesis that the Asn-43 residue in the canonical tetrahedral metal-binding site in SLC30A10 is important for Mn transport and further demonstrated that residue 43 is a key position in the protein that mediates the inward transmembrane calcium gradient for Mn exchange when expressed in HEK293T cells 90

However, further investigation is required to determine whether it also mediates copper-induced cell death

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Examples of these health conditions include those with a lack of stomach acid, those who have undergone bariatric surgery, anemia caused by the inability to absorb the vitamin, other autoimmune digestive diseases, as well as several medications