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doi: 10.1042/bj2500713

Graphical Abstract Similar content being viewed by others Abbreviations iCMs: Induced cardiomyocytes GMT: Gata4, Mef2c, Tbx5 MEFs: Mouse embryonic fibroblasts RF: RepSox + Forskolin Psr: Psoralen RF + Psr: RepSox + Forskolin + Psoralen CM: Cardiomyocyte differentiation medium Ryr2: Ryanodine receptor 2 Tnnt2: Troponin T2, cardiac type Gata4: GATA binding protein 4 -MHC: Beta-myosin heavy chain Myh6: Myosin heavy chain 6 Myl7: Myosin light chain 7 Nkx2.5: NK2 homeobox 5 cTnI: Cardiac troponin I cTnT: Cardiac troponin T -actinin: Alpha-actinin Vimentin: Intermediate filament protein marker of fibroblasts TEM: Transmission electron microscopy OCR: Oxygen consumption rate ECAR: Extracellular acidification rate 2-DG: 2-Deoxy-glucose FCCP: Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone Eto: Etomoxir PPAR: Peroxisome proliferator-activated receptor PPAR: Peroxisome proliferator-activated receptor alpha RXRG: Retinoid X receptor gamma UCP1: Uncoupling protein 1 Drp1: Dynamin-related protein 1 Fis1: Fission 1 Opa1: Optic atrophy 1 Mfn1/Mfn2: Mitofusin 1/Mitofusin 2 MFI: Mean fluorescence intensity EF: Ejection fraction FS: Fractional shortening LVIDd/s: Left ventricular internal dimension at diastole/systole LVPWs: Left ventricular posterior wall thickness at end-systole SV: Stroke volume MI: Myocardial infarction LVADs: Left ventricle assist devices Acknowledgements The authors declare that they have not used AI-generated work in this manuscript