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For cells treated with the RT-AM, real-time ratiometric images of the rapid changes of intracellular GSH concentrations in single cells can be generated by dividing the fluorescence intensity values for the 405 nm channel by the 488 nm channel at each corresponding pixel [1]

Available online at: paper-diagnostics-market (accessed July 14, 2019)

Yet, these derivatives require electron ionization (EI) which lacks sensitivity

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Folic Acid and the Prevention of Birth Defects: 30 Years of Opportunity and Controversies