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Western blot The specimens were lysed in radio immunoprecipitation assay buffer (RIPA buffer) (Cyto matin gene, Iran) with a phenyl methyl sulfonyl fluoride (PMSF) protease inhibitor (Sigma, USA) and centrifuged (15,000 rpm, 15 min, 4 C)

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Discard if expired: If the water has expired, it should be discarded, as it may no longer be sterile or effective

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