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Mutated or non-functional p-53 have been found in more than 50% human cancers leading to apoptotic resistance and continued proliferation (Kim and An, 2016)
At 16 h post-transfection, cells were collected, cryogenically snap-frozen into aliquots to ensure consistent performance across screens and seeded into 1536-well plates pre-spotted with test compounds at 4050% cell confluency in 5 L
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