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Measurement of glyoxalase activities
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PCR was carried out using 10 mM of each primer and 1ul of genomic DNA as template in 15 l reactions containing 10X Kapa Taq buffer A, 0.2 mM dNTPs, 1.5 mM MgCl 2 , 1U Kapa Taq (Kapa biosystems)
