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To remove the strong acid and base, the samples were diluted with 10 mL of a methanol:water (1:1) solution and concentrated to a final volume of 1 mL using an Amicon 3 kDa MWCO ultra centrifugal filter (Millipore, catalog no
Wilson BG, Wang X, Shen X, McKenna ES, Lemieux ME, Cho Y-J, et al
