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GO enrichment analysis indicated that these DEGs were evidently enriched in various cellular and biological processes, including organelle fission, nuclear division, mitochondrial matrix, mitochondrial inner membrane, and so on (Fig

10.1111/j.1440-1843.2012.02255.x Respirology 22 CaiS.LiZ.WangJ.WangQ.ChenR

Phosphoproteomics and total proteomics Sample preparation for MS analysis All MS experiments were performed in biological quadruplicates

Site-directed mutagenesis Plasmids encoding Flag-tagged MFAP2 wild-type (Flag-MFAP2-WT), His-tagged ITGB8 wild-type (His-ITGB8-WT), and His-tagged ITGB8 ARG57 mutant (His-ITGB8-ARG57) were transiently transfected into the cells using Lipofectamine 3000 (Thermo Fisher Scientific, Pittsburgh, PA, USA)
