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For gene expression analysis, 1 g RNA was used for cDNA synthesis using a qScript cDNA Synthesis kit (Quanta Bio, 66196756)

Changes in glutathione in acute and chronic alcohol intoxication 3

These cells secrete inhibitory cytokines such as TGF- and IL-10, and express immune checkpoint molecules such as PD-L1, jointly weakening the effector functions of cytotoxic CTLs and natural killer cells, thereby promoting tumor immune escape [88]