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Description
The glutathione assay method utilizes an adaptation of Ellmans Reagent (DTNB), where 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) reacts with GSH to form the compound TNB (2-nitro-5-thiobenzoic acid), which is then quantified using HPLC analysis
Dermatology 63 (6), 10771082
External Use Only
While Nrf2 typically induces cytoprotective gene expression, its excessive activation in psoriasis skin may paradoxically promote keratinocyte proliferation, thus contributing to disease development [46]
Supernatants were filtered over 0.2 m filters and stored at -20C until analysis
