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Right hippocampus was used for immunohistochemical studies to evaluate astrocyte and microglial reactivity focusing on the stratum radiatum of CA1 region according to our previous studies (Ezquer et al., Quantification of mRNA Levels of Nrf2 Regulated Genes in Hippocampus Twenty-four hours after the oral administration of NAC, ASA, NAC + ASA, or saline, for 11 consecutive days, chronically ethanol-consuming rats were anesthetized with chloral hydrate (280 mg/kg, i.p.) and euthanized to obtain hippocampal samples, as indicated above

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