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Angewandte Chemie

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Biochemistry 51 , 68046815 (2012)

Site-directed mutagenesis, gene synthesis, and cloning Point mutations were introduced by PCR with Pfu polymerase (Promega) using the mutagenesis primers listed in Supplementary Table 10 and the double stop-codon construct of pQE30/ SCGRX7 (ref

While this suppression diminishes NF-B activation and subsequent pro-inflammatory mediator generation, irisin conversely activates the SIRT1-p53-SLC7A11/GPX4 axis to preserve redox balance and inhibit ferroptosis (116)