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This dosing regimen (20 mg at 23:00 h and 10 mg at 07:00 h) was evaluated in the extended pharmacokinetic study in healthy volunteers and was shown to provide consistent physiological levels of hydrocortisone with similar relatively bioavailability to immediate-release hydrocortisone, and the serum concentration was observed to increase linearly with doses between 5 and 30 mg [12]

3-amino-benzoic acid (50 M) was added to each sample as internal standard prior to UPLC-MS analysis

51 SinghA.KukretiR.SasoL.KukretiS

doi: 10.1016/j.freeradbiomed.2021.01.055

[DOI] [PMC free article] [PubMed] [Google Scholar] 193.Arch JR, Ainsworth AT, Cawthorne MA, et al