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K-means clustering Five clusters were used for k-means clustering, per the results of the sum of squared estimate of errors curve, on the ASV relative abundance data with clustering by animal (n = 25)

2.2 Study participants Participant enrollment occurred from April 2024 to July 2024

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BAC 3ml vs bacteriostatic 0.9% NaCl : same preservation principle (benzyl alcohol) but plasma-isotonic solution (154 mmol/L NaCl)

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