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NAD/NADH and NADP/NADPH determination The content of nicotinamide adenine dinucleotides was determined using enzyme cycling assays, employing 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) as the terminal electron acceptor, with phenazine methosulfate (PMS) serving as an electron carrier

All animal protocols have been approved by the UNTHSC committee for animal research

Furthermore, we tested the lysosomal injury using acridine orange, an acidophilic dye that stains lysosomes 15

Although NRPS product assembly frequently seems to display a strict correlation between the enzymatic domain sequence of the megasynthase and the position of the amino acid building blocks in the peptide product, this colinearity rule is not strictly reinforced in nature 36,37