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After 0 h, 24 h, 48 h, 72 h, and 96 h of inoculation, the CCK-8 reaction solution was added according to the instructions of the CCK-8 kit (Beyotime, Shanghai, China), and the absorbance value at the wavelength of 450 nm was detected using an TECAN reader after incubation at 37 C for 1 h

Comparing outcomes from a localized SubQ injection (lumbar/glute) versus a distant SubQ injection (abdomen) can yield powerful data on the compound's site-enhancement effects

Collier, A

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Petraglia A., Plog B., Dayawansa S., et al., The Pathophysiology Underlying Repetitive Mild Traumatic Brain Injury in a Novel Mouse Model of Chronic Traumatic Encephalopathy, Surgical Neurology International 5 (2014): 184, 10.4103/2152-7806.147566