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It results in a higher percentage of degraded RBCs, thereby reducing the RBC count and causing anemia during sepsis (eryptotic RBCs were degraded and cleared from circulation) [202, 230, 380]

[DOI] [PMC free article] [PubMed] [Google Scholar] Ou Q, and King-Jones K (2013)

However, Gid12, rigidly affixed to Gid4 and Gid5, would partially occupy the space between Gid4 and the RING domains and could limit substrate accessibility of a bulky substrate to a ubiquitylation active site

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The protein samples were subjected to SDS-PAGE electrophoresis and transferred onto a nitrocellulose membrane (GE Healthcare)