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doi: 10.1152/ajpcell.00015.2022

After the ethanol was removed the next day, the cells were re-suspended in ice-cold PBS, treated with RNAse A (Keygen Biotech, China) at 37 C for 30 min, and then incubated with the DNA staining solution propidium iodide (PI, Keygen Biotech, China) at 4 C for 30 min

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This line expresses very high levels of hTERT mRNA and telomerase and was used to construct a standard curve for telomerase activity