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Culture-Independent Approach Besides that, different approaches have been developed to study the microbiome, including clone libraries, terminal restriction fragment length polymorphism (T-RFLP), quantitative PCR (qPCR), transcriptome microarrays, as well as high throughput sequencing technologies (Illumina sequencing and 454 pyrosequencing)

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Our young group represents a human age equivalent of 20-30 years, which is considered skeletally mature, and our aged group represent a human age equivalent of 55-65 years of age, a clinically relevant population for tendon degeneration 68