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A construct of pcDNA3.1(+) GSTP1 containing a V5 tag at the COOH-terminus [pcDNA3.1(+)-GSTP1_V5] was generated by PCR amplification of pcDNA3.1(+)-GSTP1 using the forward primer 5TACCGAGCTC GGATCC GCCATGCCGCCGTATACCGTG 3with a BamHI restriction site indicated in italics and the GSTP1 sequence in bold

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Alibhai FJ, Reitz CJ, Peppler WT, Basu P, Sheppard P, Choleris E, Bakovic M, Martino TA (2018) Female Clock19/19 mice are protected from the development of age-dependent cardiomyopathy