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Generation of the IscE1 meganuclease-based Tg( tg:nrf2a_T2A_mKO2nls ) transgenic zebrafish line The Gateway cloning technologybased Tol2kit (Kwan et al, 2007) was used to generate a rescue construct in which the functional nrf2a coding sequence fused to a nuclear-localized mKO2 fluorescent protein with a viral T2A linker peptide (Kim et al, 2011) is expressed under the control of a fragment of the zebrafish tg gene promoter previously shown to allow thyroid-specific transgene expression (Opitz et al, 2012)

Iron also increases the formation of non-normal fibers, a major event in PD (Abeyawardhane et al., 2018)

However, because it is an amino acid derivative, some practitioners recommend taking it on an empty stomach (or at least away from heavy, protein-rich meals) to prevent it from competing with other dietary amino acids for absorption in the intestinal tract

Int J Endocrinol

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