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[DOI] [PMC free article] [PubMed] [Google Scholar] 33.Mikkelsen K., Apostolopoulos V

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Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR) for TGF-1 mRNA Total RNA was extracted from frozen lung tissues with TRIzol reagent (TaKaRa Biotechnology Co., Ltd., Dalian, China) and was reverse-transcribed using a reverse transcription kit (TaKaRa Biotechnology Co., Ltd., Dalian, China)

The MGBA involves complex interactions between multiple systems such as the nervous system, endocrine system, and immune system