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10.1034/j.1398-9995.2003.00264.x 17

The acanthocytes are due to a defect in the lipid component of the membrane

In simple terms, a dogs immune system mistakes something in the gut (like food, bacteria, or even its own cells) as a threat, launching an attack that results in inflammation

After establishing this line, it was grown and expanded further in RPMI 1640 media supplemented with 10% fetal bovine serum
