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The AMPK pathway becomes active in response to insufficient cellular ATP levels which then leads to mTORC1 inhibition and diminished glutamine transporter expression

It also significantly reduces androgen levels in the ovaries and adrenal glands potentially mitigating the reproductive and metabolic complications related with PCOS [165]

Testosterone pellets provide steady, long-acting hormone support by maintaining consistent testosterone levels over time

actual dose females: 0.1, 1, 14, 143, 1430 g/kg bw, respectively 1.6, 16, 199, 1988, 19,880 nmol/kg bw) (Supplementary Table S1)

Finally, MBP-His-SDJ1 was added to each tube and incubated in PBSN buffer at 4 C for 2 h