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In summary, ferroptosis regulates the immune microenvironment in GBM through multiple mechanisms: promoting DC activation and CD8+ T-cell responses, modulating the direction of TAM polarization, maintaining the immune suppression stability of Tregs, and potentially enhancing immune escape through MDSCs

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Cells were incubated in Hank's buffer with 2 M MitoSox-Red for 30 min at 37 C in a 5% CO 2 atmosphere, washed with PBS and the fluorescence assessed by flow cytometry or fluorescence microscopy

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