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Every 5th fraction was combined into a new Eppendorf to make five final fractions that were dried using a SpeedVac vacuum concentrator

Hydroxocobalamin, the form most used in the UK, is released gradually, and a maintenance dose is often given every eight to twelve weeks

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Muoz-Calvo MT, Barrios V, Pozo J, Chowen JA, Martos-Moreno G, Hawkins F, Dauber A, Domen HM, Yakar S, Rosenfeld RG, Prez-Jurado LA, Oxvig C, Frystyk J, Argente J