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The supernatant was discarded and the pellet gently washed three times with phosphate buffer (50 mM phosphate buffer, pH 7.5)

Studies examining tinnitus localization revealed that approximately half of the affected individuals perceived the sound centrally in the head or bilaterally, while the remainder most often reported left-sided tinnitus, 33 and a minority of patients believed that the tinnitus originated from an external source

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9,13 Thyroid stimulating hormone (TSH)

Therefore, future research on the dynamics of ferroptosis in motor neuron degeneration of ALS models such as SOD1 G93A mice is clearly warranted