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The M1 resin was pack into a column and washed with 20 column volumes of 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 3 mM CaCl 2 , 0.01% (w/v) LMNG and 0.0006% (w/v) CHS, 1 M peptide
The serum concentration will vary with the cell line and basal medium used
