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For each reaction, reverse transcription was performed at 25 for 5 min, followed by an incubation at 50 for 15 min and at 85 for 5 min

10.3389/fimmu.2021.715723 26 KockA.SchwarzT.KirnbauerR.UrbanskiA.PerryP.AnselJ

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The physiologically active form of the medium-chain acylcarnitine octanoyl-L-carnitine is a marker of medium-chain acetyl-CoA dehydrogenase deficiency, and is also a marker in the ratios of -ketothiolase deficiency, holocarboxylase synthesis, biotinidase, and glutaric acidemia