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In a 2019 study, researchers developed an oral DNA vaccine using attenuated Salmonella typhimurium to deliver a plasmid encoding both IL-2 and the VP60 capsid protein of rabbit hemorrhagic disease virus (RHDV) ( A DNA vaccine co-expressing chicken IL-2 (chIL-2) and IL-7 (chIL-7) with the VP2 antigen demonstrated enhanced immunogenicity and protective efficacy against infectious bursal disease (IBDV) in a 2019 study ( Edwardsiella tarda ( Similarly, co-delivery of chicken IL-2 (chIL-2) with an infectious laryngotracheitis virus (ILTV) chicken embryo origin (CEO) vaccine significantly alleviated vaccine-induced clinical signs without compromising protective efficacy ( Finally, a 2025 study reported a multi-component DNA-launched plasmid prevented autoimmune diabetes in nonobese diabetic (NOD) mice (57)