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Immunohistochemical staining of the tight junction-associated protein ZO-1 was also conducted for bEND.3 cells before and after exposure to FUS, using anti-ZO-1 rabbit polyclonal antibodies and TRITC-conjugated anti-rabbit IgG 37

Protein precipitation using methanol (Merck KGaA, Darmstadt, Germany) and 0.1% formic acid (Merck KGaA, Darmstadt, Germany) was performed and the collected supernatant was treated with a derivatization solution of aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) (Merck KGaA, Darmstadt, Germany)

Human metabolism and renal excretion of selenium compounds after oral ingestion of sodium selenite and selenized yeast dependent on the trimethylselenium ion (TMSe) status

& Sonsuz, A

B Biointerfaces 245, 114243