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The participation of transition metal ions in the free radical generation is protected by several metal-sequestering proteins (e.g

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Nocito A and others demonstrated that TPH1 knock-out mice had significantly less severe liver injury after ischemia/reperfusion, related to the accelerated hepatocyte proliferation in the knock-out mice 16

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Organoids were transferred to a 6 well-plate on a CO 2 resistant orbital shaker (Thermo Scientific) and grown in differentiation medium consisting of DMEM:F12 supplemented with 1X N2 supplement, 1X B27 supplement, 1X penicillin (100 units/mL)/streptomycin (100 g/mL), 0.1mM 2-mercaptoethanol, 1X NEAA, and 2.5 g/mL human insulin