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Vitiligo is a chronic autoimmune disease in which the bodys immune system mistakenly attacks pigment-producing cells (melanocytes)

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Representative tissue specimens were collected, dehydrated and embedded in paraffin blocks, sectioned at 4 m, and stained with hematoxylin-eosin according to the following automated Sakura Tissue-Tek DRS 2000 Slide Stainer protocol ( accessed on 19 October 2021): rehydration in distilled water, staining with hematoxylin, washing in running tap water, differentiation with 70% alcohol, staining with eosin, dehydration, clearing, and mounting

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Forward and reverse primer sequences used for quantitative real-time PCR (qRT-PCR), along with annealing temperatures for each target gene