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For a 150 min run, peptides were eluted over a 150 min gradient using 80% acetonitrile, and 0.1% formic acid (buffer B), going from 2% to 8% over 10 min, to 45% over 115 min, then to 65% over 20 min, and then kept at 95% for 10 min, after which all peptides were eluted

Anesthesiology 84 , 1011 (1996)

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