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Binding of a single zinc ion to one subunit of copper-zinc superoxide dismutase apoprotein substantially influences the structure and stability of the entire homodimeric protein
[DOI] [PubMed] [Google Scholar] 84.Reeder S.B., Yokoo T., Frana M., Hernando D., Alberich-Bayarri ., Alstiza J.M., Gandon Y., Henninger B., Hillenbrand C., Jhaveri K., et al

Lets imagine that you are trying to express and purify a new protein
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