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This heightens susceptibility to the toxic effects of iron overload, disrupting intestinal mucosal homeostasis and triggering local inflammation and cell death, ultimately precipitating the onset of gut-associated diseases ( Iron overload-related diseases rank among the most prevalent genetic disorders in humans, characterized pathologically by systemic iron accumulation resulting from excessive dietary iron absorption and iron-induced oxidative stress responses ( 3 Ferroptosis and inflammatory bowel disease The imbalance in ferroptosis regulation plays a key role in the pathogenesis of various diseases

This protection is especially important for sensitive tissues, such as those in the brain

However, there is a notable lack of formal safety data

The final common effect of these molecular pathways seems to be represented by increased oxidative stress.7 The alteration of the cellular oxidant-antioxidant status is able to induce a diffuse endothelial dysfunction and contribute to the progressive development of micro- and macrovascular complications (Figure 1).8 Figure 1 Schematic representation of oxidative stress pathways

Therefore, this metabolic microenvironment of solid tumors can be recapitulated, and throughout the study we used 0.5 mM glucose in media to recapitulate glucose deprivation and, on top of this, added 20 mM lactic acid for a mimicry of lactic acidosis