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Cells were subsequently washed with pre-warmed BD Pharmingen Permeabilization Buffer, centrifuged, and incubated in the same buffer for 30 minutes at 37C in the dark for permeabilization

doi: 10.1210/jc.2010-2679 47 HjelholtAJLeeKYArlien-SborgMCPedersenSBKopchickJJPuriVet al

The gut-healing effects, while well-documented in animals, also lack robust human trial data, though anecdotal reports from clinical use are consistently positive

[39:10] Andrew Huberman And I will say, because for people that think it's just bro science, it's also gal science

It has been assigned Generally Recognized As Safe (GRAS) status by the FDA for food, beverage, and dietary supplement applications