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Note on humans: Beyond Barth syndrome these benefits are not established major trials in mitochondrial myopathy (MMPOWER-3) and heart failure (PROGRESS-HF) failed their primary endpoints [4] [5]

Approximately 60 % of marketed compounds are cleared by hepatic cytochrome P450 (CYP)-mediated metabolism 1 Liver microsomes are subcellular fractions which contain membrane bound drug metabolizing enzymes such as CYP Microsomes can be used to determine the in vitro intrinsic clearance of a compound The use of species-specific microsomes can be used to enable an understanding of interspecies differences in drug metabolism Microsomes are easy to prepare, use and store enabling cost efficiencies over whole cell models Microsomes are pooled from multiple donors to minimize the effect of interindividual variability Microsomes are fully characterized using probe substrates to ensure activity is maintained between batches Protocol Microsomal Stability Assay Protocol Follow on metabolite profiling and structural elucidation: Cyprotex's microsomal stability assay can be extended to profile the metabolites that are formed

Simpson EP, Henry YK, Henkel JS, Smith RG, Appel SH